A dietary intervention study building on the results from the first geoMOUSE project. Here, one of three isocaloric diets of varying macronutrient compositions are given to male mice, which are then mated to produce offspring. This study aims to extend the investigation on whether offspring epigenetic profiles are influenced by paternal nutrition and how this affects behavioral and cognitive outcomes.
TEs (Transposable Elements), escape epigenome reprogramming and therefore represent potential hotspots of heritable information that can be passed on to future generations. The Latin suffix –theca (from Ancient Greek thēkē) is used for any kind of collection. The goal of the GeckoTEk project is to generate a near-complete human sperm (epi)genome of high-quality, using third-generation sequencing technologies with a particular focus on difficult genomic regions and repetitive sequences.
An ex vivo characterisation of tissue specific epigenetic remodelling in offspring sired from nutritionally challenged fathers. We utilise approaches such as HI-C and ATAC-seq to develop a picture of genetic architecture, and integrate chromatin confirmation and transcriptomic data to determine how epigenetic regulatory elements reshape the genome.
EpiPIG is a research study in which we are giving mini-pigs either a ‘Western’ high fat/high sugar diet or a normal pig diet to better understand what epigenetic impact this has on their sperm.
A human dietary intervention study where participants receive different commonly consumed diet patterns and we are examining the impact it has on epigenetic markers in the sperm. This part of the study is with individual people, singletons.
A human dietary intervention study where participants receive different commonly consumed diet patterns and we are examining the impact it has on epigenetic markers in the sperm. This branch of the study has twin male participants.
A dietary intervention study in which male mice are given one of 10 diets with different proportions of protein, fat and carbohydrates, and then mated to produce offspring. Following which, we examine the effect of these different diets on the overall health and behaviour of both the males and their offspring. We are especially looking for an epigenetic patterns.
A dietary intervention study with guinea pigs fed high or low –fat diets with or without additional Vitamin C to identify through which mechanisms nutritional factors influence epigenetic inheritance of obesity and metabolic disease.
A partnership with Taronga Zoo and the Copenhagen Zoo to assess sperm epigenetic signatures across a wide range of species. The study aims to build a reference map of sperm epigenome modifications among animal species to understand similarities and differences in what environmental information is transmitted in sperm.
SEAS = Sperm Epigenomics Across Species
Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots.
We exposed young men to a 6-week endurance training exercise regime, and measured the epigenetic signature of their sperm before and after the intervention. This study highlighted exercise-induced remodelling of genes involved in the brain.
The epigenetic signature of both lean and obese men, and men before after weight loss was examined, highlighting both rapid and long-term remodelling of the sperm epigenome at gene regions involved in appetite regulation.
